The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. Temperature control: 4°C above ambient to 45°C. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 303 unable to cal dark current. Experience the identifiably different. Microplates for applications that require high sample recovery rates. –Min. Integrated stacker option. plates < 100 amol (384-well) 1 0200-7019. MultiWash+ Microplate Washer. plates < 100 amol (384-well) 1 0200-7018. Ensure data accuracy of your reader. 58% for the detection module and 2. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. RUO. Molecular Devices: n/a:. com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 2 with 1000 cells. 48, 96, and 384 wells can be used in the SpectraMax M2 and SpectraMax M2e. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Application Area: Spectrophotometer for molecular biology. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. 2. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. 2. Streamline your workflow with comparative confluence assays to identify and verify gene edits. Related products. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Suggested plates are black. Dynamic range > 6 decades . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 96 deep well plate: packed in sleeves of 6 , 24 per case. In addition, SoftMax Pro SoftwareThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. use as little as 2 µL samples with 24- or 64-well plates. ©2010 Molecular Devices, Inc. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Kinetic assays were read every 20-28 seconds for one hour,measurements in compatible Molecular Devices® microplate readers. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Published Dec 16, 2020 | Updated Nov 07, 2022. It has a. x. Outer wells aligned for proper viewing with FLIPR Ideal. The SpectraMax 190 reader can be integrated with Molecular . U-bottom: 30-40 µl. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. It is more inert and reduces compound interference. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Browse for different fluorescent readers below and compare across different instrument suppliers. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. With optimized reagents, option to operate the readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Gemini XPS Reader scans. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. x. 3. A. To update the driver. Documentation:Molecular Devices microplate readers. Round well design with a clear flat bottom 2. Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. 3 µl). Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. tools to expand the boundaries of their research. 0 OD for earlier models. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Recertification service. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. The GenePix® Microarray Systems are based on 25 years of expertise in low-noise signal amplification and optical design. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Supplier: Molecular Devices. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. Two different agonists, acetylcholine and carbachol, were tested. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 96/384-well plate formats. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. FlexIII. the Gemini XPS reader. The FLEXstation II 384 (Molecular Devices, Inc. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. 20 attomol ATP per well. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. 020 fmol 0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 8 mm distance between well bottoms to plate bottom. 305 reference level saturation. San Jose, CA 95134. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 20 attomol ATP per well. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. B. B. Can read 96 well plates (optional 384 well). “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. These lab workhorses are used in drug discovery, research, bioassay validation, and. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. 2. This enhanced capability. begin reading microplates with seven mouse clicks. Backed by the. Catalog number: AB1384W. Test up to 3. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535 (Molecular Devices P/N 5089097). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 96-1536. Fluorescent Polarization: Solid black plates, as above. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. Extended linear dynamic range. In addition, the iD5 reader measures TRF and FP and. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Comprehensive solution. Versa Max. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Molecular Devices 大中华区 Email: info. Dual injectors for kinetic assays. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Versa Max. UV absorbance detection. Washers configured for both 96- and 384-well plates. Shaking: Linear and Orbital. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. 00. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. 6-384. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Pro, Dual Read mode was selected. 1. 4 Equipment. Category 2 and 3 microplate readers use gradient heating as well. A ten-fold serial dilution of purified luciferase in PBS with 0. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. Microplates for applications that require high sample recovery rates. The microplate was then placed in a SpectraMax. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. 7. x. FlexIII. Devices’ StakMax® Microplate Stacker in a matter of minutes and . This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Full-skirted standard or extra. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The semi-automated PX1 PCR Plate Sealer delivers reliable uniform sealing of a range. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. M2. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. CO 2 & O 2 control. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. For more sample throughput, the system canabsorbance plate readers. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Infinite possibilities. 2. Source plate capacity; 1 x plates. The plate reader optics are tunable for. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. That explains the lower values on the LmaxII384 plate reader. Access this by going to. x. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 00. The analyzed results are. Catalog number: BC1384W. Get Quote . Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. , April 14, 2022 – Molecular Devices, LLC. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The equation that allows one to calculate absorbance from % transmittance is. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. (or plate reader). VIS absorbance detection. a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. ) 0. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Ideal for miniaturized 384 well fluorescent. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 2. #PLUS 384) UV-transparent 96-well microplates (Corning cat. 384-WELL SBS STERILE MICROPLATE. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. M5. 2. Fluorescence Microplate Reader Comparison Chart. Not for use in diagnostic procedures. 003 fmol 0. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. MultiWash+ Microplate Washer. Cross-talk <0. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384. These models can no longer be repaired. 96-well fluorescent cell dilution series, top-read. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. Gemini EM Reader scans. Browse for different fluorescent readers below and compare across different instrument suppliers. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. x. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. After 30 minutes the plate was read in Analyst GT with varying z heights. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. or use the modified protocol below for the generation of NPCs (see Note 1). 384- or 96-well read plate containing cells loaded. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. • Easy and gentle immobilization of biotin. For more sample throughput, the system canThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 100 fmol luciferase per well. Note: These settings are based on necessary cookies. 4 decades . 1. 384-Well Microarray/High Sample Recovery Plate. Plate Formats: 6- to 384-well plates. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. 7°C (gradient heating design). FLIPR Calcium 4 Assay Kit. 2. 1 a. x. #6590-0118) Filter 665/10 (Molecular Devices cat. X. Summary of Contents for Molecular Devices SpectraMax M2. Other technologies such as imaging. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. Molecular Devices has provided scientists with tools to expand the boundaries of their research. x. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. x. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. 2. Others models hold the sample in place using surface tension. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. Plate formats. 2. highthroughput alphascreen, alphascreen on plate reader Created Date:SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. 0 out of 5 $ 8,495. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. Validation made easy -The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Typically, the full spectrum of signal from. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). If you have an xml plate object, then. For biochemical assays, the washer removes unbound material and unreacted reagents. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. 1/17 1907B Printed in USA . Page 154: Ordering Parts And Accessories. Filter. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. This reader also makes use of a unique temperature. 2. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. System setup and calibration are controlled from within SoftMax Pro Software. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In simplest terms, a plate reader measures and. $542. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. , San Diego, CA, USA) such that the. 1 fmol luciferase per well. XML file. x. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Total volume (except deep well plates) is 120 mL. Detection cartridge modularity allows for easy addition of new read modes within minutes without. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. )Step 4: Incubate at room temperature for 1 hour, protected from light. Time-resolved phosphorescence. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 6% for the on-board optics (monochromators). Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. x. #S1205. #6590-0144; includes Enhanced TRF Module, excitation and emission filters). 012 fmol 0. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. 00. x. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 6-384 (96- and 384-well with pipetting) 96-well microplates. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. Microplate format. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. San Jose, Calif. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Top. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Features. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Figure 3. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion.